Composite

Part:BBa_K1659501:Design

Designed by: Wei Chung Kong   Group: iGEM15_Oxford   (2015-08-28)

pLsr + RBS + sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 131


Design Notes and Sources

Sequences for BBa_K117002, BBa_B0031, and BBa_I746916 were obtained from the BioBricks Registry. An 8 bp spacer was placed between the promoter and the RBS, and a 6bp spacer was placed between the RBS and the coding sequence.